Advanced Techniques in Protein Engineering
( 25 Modules )

Module #1
Introduction to Protein Engineering
Overview of protein engineering, its importance, and applications

Module #2
Protein Structure and Function
Review of protein structure, protein-ligand interactions, and enzymology

Module #3
Molecular Biology Tools for Protein Engineering
PCR, cloning, mutagenesis, and gene expression in protein engineering

Module #4
Directed Evolution and Mutagenesis
Introduction to directed evolution, random mutagenesis, and site-directed mutagenesis

Module #5
High-Throughput Screening Methods
Overview of HTS methods, including FACS, yeast display, and phage display

Module #6
Computational Tools for Protein Engineering
Introduction to bioinformatics tools, including Rosetta, PyRosetta, and FoldX

Module #7
Rational Design and Molecular Modeling
Using computational methods to design protein mutants and predict protein-ligand interactions

Module #8
Protein-Protein Interactions and Interface Design
Designing protein-protein interactions and interfaces for novel functions

Module #9
Enzyme Engineering and Biocatalysis
Designing enzymes for novel reactions, substrate specificity, and improved catalytic efficiency

Module #10
Protein-Based Biosensors and Diagnostics
Designing protein-based biosensors for detection and diagnostics

Module #11
Protein-Nucleic Acid Interactions and Engineering
Designing protein-nucleic acid interactions for gene regulation and RNA-targeting therapies

Module #12
Computational Protein Design and De Novo Design
Designing novel proteins from scratch using computational methods

Module #13
Protein Folding and Misfolding
Understanding protein folding and misfolding, and its implications for protein engineering

Module #14
Protein Stability and Half-Life Engineering
Designing proteins with improved stability and half-life

Module #15
Glycoengineering and Glycoprotein Design
Designing glycoproteins with novel glycans and glycan structures

Module #16
Protein-Polymer Conjugates and Nanoparticles
Designing protein-polymer conjugates and nanoparticles for drug delivery and diagnostics

Module #17
Advanced Analytical Techniques in Protein Engineering
Using techniques like mass spectrometry, NMR, and cryo-EM for protein characterization

Module #18
Protein Engineering for Biotechnological Applications
Case studies of protein engineering for biotechnological applications, including biofuels and bioremediation

Module #19
Protein Engineering for Therapeutic Applications
Case studies of protein engineering for therapeutic applications, including immunotherapy and gene therapy

Module #20
Intellectual Property and Regulatory Considerations
Understanding IP and regulatory considerations for protein engineering products

Module #21
Emerging Trends and Future Directions in Protein Engineering
Exploring emerging trends and future directions in protein engineering, including CRISPR-Cas and synthetic biology

Module #22
Case Studies in Protein Engineering
In-depth case studies of successful protein engineering projects

Module #23
Computational Project:Rational Design of a Protein
Hands-on computational project on rational design of a protein

Module #24
Wet Laboratory Project:Expression and Purification of a Protein
Hands-on wet laboratory project on expression and purification of a protein

Module #25
Course Wrap-Up & Conclusion
Planning next steps in Advanced Techniques in Protein Engineering career

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Advanced Techniques in Protein Engineering ( 25 Modules )

Advanced Techniques in Protein Engineering
( 25 Modules )