Biophysical Methods in Protein Engineering
( 25 Modules )

Module #1
Introduction to Protein Engineering
Overview of protein engineering, importance of biophysical methods, and course objectives

Module #2
Biophysical Principles
Review of thermodynamics, kinetics, and spectroscopy principles relevant to biophysical methods

Module #3
UV-Vis Spectroscopy
Principles and applications of UV-Vis spectroscopy in protein engineering

Module #4
Circular Dichroism (CD) Spectroscopy
Principles and applications of CD spectroscopy in protein engineering

Module #5
Fluorescence Spectroscopy
Principles and applications of fluorescence spectroscopy in protein engineering

Module #6
Intrinsic Fluorescence
Using intrinsic fluorescence to study protein structure and dynamics

Module #7
Extrinsic Fluorescence
Using extrinsic fluorescence to study protein-ligand interactions and structural changes

Module #8
Nuclear Magnetic Resonance (NMR) Spectroscopy
Principles and applications of NMR spectroscopy in protein engineering

Module #9
Chemical Shift and Assignment
Using chemical shift and assignment to determine protein structure and dynamics

Module #10
Relaxation and Dynamics
Using NMR relaxation and dynamics to study protein motion and flexibility

Module #11
Electron Paramagnetic Resonance (EPR) Spectroscopy
Principles and applications of EPR spectroscopy in protein engineering

Module #12
Mass Spectrometry (MS)
Principles and applications of MS in protein engineering, including protein identification and characterization

Module #13
Surface Plasmon Resonance (SPR)
Principles and applications of SPR in protein engineering, including protein-ligand interactions and kinetics

Module #14
Isothermal Titration Calorimetry (ITC)
Principles and applications of ITC in protein engineering, including protein-ligand interactions and thermodynamics

Module #15
Differential Scanning Calorimetry (DSC)
Principles and applications of DSC in protein engineering, including protein stability and thermodynamics

Module #16
Size Exclusion Chromatography (SEC)
Principles and applications of SEC in protein engineering, including protein size and interactions

Module #17
Multi-Angle Light Scattering (MALS)
Principles and applications of MALS in protein engineering, including protein size and interactions

Module #18
Computational Tools for Biophysical Analysis
Overview of computational tools for biophysical data analysis, including molecular modeling and simulations

Module #19
Molecular Dynamics Simulations
Principles and applications of molecular dynamics simulations in protein engineering

Module #20
Molecular Docking and Virtual Screening
Principles and applications of molecular docking and virtual screening in protein engineering

Module #21
Biophysical Methods in Protein Design
Applications of biophysical methods in protein design, including enzyme engineering and protein-protein interactions

Module #22
Biophysical Methods in Protein Evolution
Applications of biophysical methods in protein evolution, including directed evolution and molecular evolution

Module #23
Biophysical Methods in Protein Misfolding and Aggregation
Applications of biophysical methods in protein misfolding and aggregation, including amyloid fibrils and protein misfolding diseases

Module #24
Case Studies in Biophysical Protein Engineering
Real-world examples of biophysical methods in protein engineering, including protein therapeutics and biosensors

Module #25
Course Wrap-Up & Conclusion
Planning next steps in Biophysical Methods in Protein Engineering career

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Biophysical Methods in Protein Engineering ( 25 Modules )

Biophysical Methods in Protein Engineering
( 25 Modules )