Directed Evolution and Protein Design
( 25 Modules )

Module #1
Introduction to Directed Evolution and Protein Design
Overview of the field, importance, and applications

Module #2
Principles of Evolution and Natural Selection
Review of fundamental concepts in evolution and their relevance to directed evolution

Module #3
Protein Structure and Function
Review of protein structure, folding, and function, and their importance in protein design

Module #4
Introduction to Directed Evolution Methods
Overview of different directed evolution methods, including mutagenesis, gene shuffling, and phage display

Module #5
Mutagenesis and Gene Shuffling
In-depth exploration of mutagenesis and gene shuffling methods, including error-prone PCR and DNA shuffling

Module #6
Phage Display and Yeast Display
In-depth exploration of phage display and yeast display methods for protein engineering

Module #7
High-Throughput Screening Methods
Overview of high-throughput screening methods for protein engineering, including FACS and NextGen sequencing

Module #8
Computational Tools for Protein Design
Introduction to computational tools for protein design, including Rosetta and FoldX

Module #9
Protein-Ligand Interactions and Binding Affinity
Examining protein-ligand interactions and binding affinity in protein design

Module #10
Stability and Folding in Protein Design
Exploring the importance of stability and folding in protein design

Module #11
Enzyme Engineering and Directed Evolution
Case studies of directed evolution for enzyme engineering and applications

Module #12
Antibody Engineering and Directed Evolution
Case studies of directed evolution for antibody engineering and applications

Module #13
Protein-Protein Interactions and Directed Evolution
Exploring the role of protein-protein interactions in directed evolution and protein design

Module #14
Machine Learning and Artificial Intelligence in Protein Design
Introduction to machine learning and AI methods for protein design and optimization

Module #15
De Novo Protein Design and Rational Design
Exploring de novo protein design and rational design approaches

Module #16
Protein Engineering for Biotechnological Applications
Case studies of protein engineering for biotechnological applications, including biofuels and bioproducts

Module #17
Protein Engineering for Therapeutic Applications
Case studies of protein engineering for therapeutic applications, including cancer and infectious diseases

Module #18
Ethical Considerations in Protein Design and Directed Evolution
Examining the ethical implications of protein design and directed evolution

Module #19
Intellectual Property and Patenting in Protein Design
Overview of intellectual property and patenting issues in protein design

Module #20
Experimental Design and Statistical Analysis in Directed Evolution
Best practices for experimental design and statistical analysis in directed evolution experiments

Module #21
Case Studies in Directed Evolution and Protein Design
In-depth examination of successful case studies in directed evolution and protein design

Module #22
Challenges and Limitations in Directed Evolution and Protein Design
Examining the challenges and limitations of directed evolution and protein design

Module #23
Emerging Trends and Future Directions in Directed Evolution and Protein Design
Exploring emerging trends and future directions in the field

Module #24
Computational Project:Protein Design and Directed Evolution
Hands-on computational project applying protein design and directed evolution principles

Module #25
Course Wrap-Up & Conclusion
Planning next steps in Directed Evolution and Protein Design career

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Directed Evolution and Protein Design ( 25 Modules )

Directed Evolution and Protein Design
( 25 Modules )