Introduction to Protein Engineering and Design
( 25 Modules )

Module #1
Introduction to Protein Engineering
Overview of protein engineering, its importance, and applications

Module #2
Protein Structure and Function
Review of protein structure, folding, and function

Module #3
Protein Engineering Strategies
Introduction to rational design, directed evolution, and semi-rational design

Module #4
Molecular Biology Tools for Protein Engineering
Overview of molecular biology techniques used in protein engineering, including PCR, cloning, and mutagenesis

Module #5
Protein Expression Systems
Introduction to different protein expression systems, including E. coli, yeast, and mammalian cells

Module #6
Protein Purification and Characterization
Overview of protein purification methods, including chromatography and crystallization

Module #7
Bioinformatics for Protein Engineering
Introduction to bioinformatics tools and databases for protein engineering, including BLAST and Pfam

Module #8
Rational Protein Design
Introduction to rational protein design principles, including site-directed mutagenesis and computational design

Module #9
Computational Tools for Protein Design
Overview of computational tools for protein design, including Rosetta and PyMOL

Module #10
Directed Evolution of Proteins
Introduction to directed evolution methods, including error-prone PCR and DNA shuffling

Module #11
High-Throughput Screening Methods
Overview of high-throughput screening methods for protein engineering, including phage display and yeast display

Module #12
Protein-Ligand Interactions
Introduction to protein-ligand interactions, including binding affinity and specificity

Module #13
Protein-Protein Interactions
Overview of protein-protein interactions, including protein complexes and protein networks

Module #14
Stability and Folding of Engineered Proteins
Introduction to stability and folding of engineered proteins, including thermal stability and aggregation

Module #15
Enzyme Engineering
Introduction to enzyme engineering, including enzyme kinetics and mechanism

Module #16
Antibody Engineering
Overview of antibody engineering, including antibody structure and function

Module #17
Protein Engineering for Biomedical Applications
Introduction to protein engineering for biomedical applications, including protein therapeutics and diagnostics

Module #18
Protein Engineering for Industrial Applications
Overview of protein engineering for industrial applications, including biofuels and biocatalysis

Module #19
Case Studies in Protein Engineering
In-depth analysis of case studies in protein engineering, including successes and challenges

Module #20
Ethical Considerations in Protein Engineering
Discussion of ethical considerations in protein engineering, including biosafety and biosecurity

Module #21
Current Trends and Future Directions in Protein Engineering
Overview of current trends and future directions in protein engineering, including synthetic biology and CRISPR

Module #22
Lab-Based Protein Engineering
Hands-on protein engineering exercises, including cloning, expression, and purification

Module #23
Computational Protein Engineering Project
Guided computational protein engineering project, including design, simulation, and analysis

Module #24
Group Project Presentations
Student group presentations of protein engineering projects, including design, implementation, and results

Module #25
Course Wrap-Up & Conclusion
Planning next steps in Introduction to Protein Engineering and Design career

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Introduction to Protein Engineering and Design ( 25 Modules )

Introduction to Protein Engineering and Design
( 25 Modules )