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Apprentice Mode
10 Modules / ~100 pages
Wizard Mode
~25 Modules / ~400 pages

Molecular Diagnostics Techniques
( 30 Modules )

Module #1
Introduction to Molecular Diagnostics
Overview of molecular diagnostics, history, and applications
Module #2
Principles of Molecular Biology
Review of molecular biology concepts, including DNA, RNA, and proteins
Module #3
Nucleic Acid Chemistry
Chemical properties of nucleic acids, including hybridization and amplification
Module #4
Molecular Diagnostics Techniques Overview
Survey of molecular diagnostics techniques, including PCR, sequencing, and hybridization
Module #5
Quality Control and Assurance in Molecular Diagnostics
Importance of quality control and assurance in molecular diagnostics, including regulatory considerations
Module #6
Polymerase Chain Reaction (PCR) Basics
Principles and mechanisms of PCR, including real-time PCR
Module #7
Nested PCR and Multiplex PCR
Advanced PCR techniques, including nested PCR and multiplex PCR
Module #8
Quantitative PCR (qPCR)
Theory and applications of qPCR, including gene expression analysis
Module #9
Reverse Transcription PCR (RT-PCR)
Principles and applications of RT-PCR, including cDNA synthesis
Module #10
Digital PCR
Theory and applications of digital PCR, including absolute quantification
Module #11
Sanger Sequencing
Principles and applications of Sanger sequencing, including sequencing by synthesis
Module #12
Next-Generation Sequencing (NGS) Technologies
Overview of NGS platforms, including Illumina, Ion Torrent, and PacBio
Module #13
NGS Data Analysis
Bioinformatics tools and techniques for NGS data analysis
Module #14
Targeted Sequencing
Theory and applications of targeted sequencing, including amplicon sequencing
Module #15
Whole Genome Sequencing
Theory and applications of whole genome sequencing, including genome assembly and annotation
Module #16
Southern Blotting
Principles and applications of Southern blotting, including DNA hybridization
Module #17
Microarrays
Theory and applications of microarrays, including gene expression analysis
Module #18
In Situ Hybridization
Principles and applications of in situ hybridization, including FISH and CISH
Module #19
Fluorescence In Situ Hybridization (FISH)
Theory and applications of FISH, including probe design and interpretation
Module #20
Chromogenic In Situ Hybridization (CISH)
Theory and applications of CISH, including probe design and interpretation
Module #21
Molecular Beacon Technology
Theory and applications of molecular beacons, including real-time detection
Module #22
Loop-Mediated Isothermal Amplification (LAMP)
Principles and applications of LAMP, including point-of-care diagnostics
Module #23
Nanopore Sequencing
Theory and applications of nanopore sequencing, including portable sequencing
Module #24
CRISPR-Cas9 Gene Editing
Principles and applications of CRISPR-Cas9, including gene therapy and diagnostics
Module #25
Artificial Intelligence and Machine Learning in Molecular Diagnostics
Applications of AI and ML in molecular diagnostics, including biomarker discovery and disease diagnosis
Module #26
Molecular Diagnostics in Infectious Diseases
Applications of molecular diagnostics in infectious disease diagnosis and management
Module #27
Molecular Diagnostics in Cancer
Applications of molecular diagnostics in cancer diagnosis, treatment, and monitoring
Module #28
Molecular Diagnostics in Genetic Disorders
Applications of molecular diagnostics in genetic disorder diagnosis and management
Module #29
Molecular Diagnostics in Pharmacogenomics
Applications of molecular diagnostics in pharmacogenomics and personalized medicine
Module #30
Course Wrap-Up & Conclusion
Planning next steps in Molecular Diagnostics Techniques career


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