PCR and Molecular Diagnostic Applications
( 25 Modules )

Module #1
Introduction to PCR
History, principles, and advantages of Polymerase Chain Reaction (PCR)

Module #2
PCR Basics
Components, cycling conditions, and types of PCR (end-point, real-time, multiplex)

Module #3
PCR Techniques
Conventional PCR, nested PCR, touchdown PCR, and arbitrary primed PCR

Module #4
Real-Time PCR
Principles, instrumentation, and applications of real-time PCR

Module #5
PCR Applications in Molecular Diagnostics
Infectious disease detection, genetic testing, and cancer diagnosis using PCR

Module #6
Molecular Diagnostics Overview
Definition, types, and importance of molecular diagnostics in healthcare

Module #7
Nucleic Acid Extraction and Purification
Methods and importance of high-quality DNA/RNA extraction for PCR and molecular diagnostics

Module #8
PCR Inhibitors and Troubleshooting
Common PCR inhibitors, troubleshooting strategies, and optimization techniques

Module #9
Quantification Methods in PCR
Absolute and relative quantification, standard curves, and delta-delta Ct method

Module #10
Microbial Identification using PCR
PCR-based methods for identifying bacteria, viruses, and other microorganisms

Module #11
Genetic Variation Analysis using PCR
PCR-based methods for detecting mutations, SNPs, and other genetic variations

Module #12
Cancer Diagnosis and Monitoring using PCR
PCR-based methods for cancer diagnosis, detection of minimal residual disease, and monitoring therapy response

Module #13
PCR in Forensic Science
PCR-based methods for DNA analysis in forensic investigations

Module #14
Next-Generation PCR Technologies
Digital PCR, droplet digital PCR, and other emerging PCR technologies

Module #15
Molecular Diagnostics in Infectious Diseases
PCR-based methods for detecting and characterizing infectious diseases (e.g., HIV, TB, influenza)

Module #16
Personalized Medicine and Pharmacogenomics
PCR-based methods for genetic testing and personalized medicine

Module #17
Quality Control and Quality Assurance in PCR
Importance of quality control, quality assurance, and validation in PCR-based molecular diagnostics

Module #18
Regulatory Affairs and Accreditation in Molecular Diagnostics
Regulatory frameworks, accreditation, and certification in molecular diagnostics

Module #19
Case Studies in Molecular Diagnostics
Real-world examples of PCR-based molecular diagnostics in various fields

Module #20
Emerging Trends and Future Directions in PCR
Advances in PCR technologies, nano-biotechnology, and potential applications

Module #21
PCR Instrumentation and Automation
Overview of PCR instrumentation, automation, and high-throughput screening

Module #22
Bioinformatics and Data Analysis in PCR
Introduction to bioinformatics, data analysis, and interpretation in PCR-based molecular diagnostics

Module #23
PCR in Agricultural Biotechnology
PCR-based methods for crop improvement, disease diagnosis, and food safety testing

Module #24
PCR in Environmental Monitoring
PCR-based methods for monitoring water and air quality, and detecting environmental pollutants

Module #25
Course Wrap-Up & Conclusion
Planning next steps in PCR and Molecular Diagnostic Applications career

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PCR and Molecular Diagnostic Applications ( 25 Modules )

PCR and Molecular Diagnostic Applications
( 25 Modules )