Recombinant Protein Production
( 25 Modules )

Module #1
Introduction to Recombinant Protein Production
Overview of recombinant protein production, importance, and applications

Module #2
Principles of Molecular Biology
Review of molecular biology principles, DNA, RNA, and protein synthesis

Module #3
Cloning Vectors and Plasmids
Introduction to cloning vectors, plasmids, and their uses in recombinant protein production

Module #4
Gene Isolation and Amplification
Methods for gene isolation and amplification, including PCR and gene synthesis

Module #5
Construct Design and Vector Construction
Designing and constructing expression vectors for recombinant protein production

Module #6
Host Organisms for Recombinant Protein Production
Overview of host organisms used in recombinant protein production, including E. coli, yeast, and mammalian cells

Module #7
Transformation and Transfection Methods
Methods for introducing recombinant DNA into host organisms, including transformation and transfection

Module #8
Selection and Screening of Recombinant Clones
Methods for selecting and screening recombinant clones, including antibiotic selection and colony PCR

Module #9
Fermentation and Cell Culture
Overview of fermentation and cell culture for recombinant protein production, including media and process conditions

Module #10
Cell Lysis and Protein Extraction
Methods for cell lysis and protein extraction from recombinant host organisms

Module #11
Protein Purification Methods
Overview of protein purification methods, including chromatography, precipitation, and centrifugation

Module #12
Protein Analysis and Characterization
Methods for protein analysis and characterization, including SDS-PAGE, Western blot, and mass spectrometry

Module #13
Quality Control and Validation
Importance of quality control and validation in recombinant protein production, including regulatory requirements

Module #14
Scale-Up and Process Optimization
Strategies for scaling up recombinant protein production and optimizing process conditions

Module #15
Troubleshooting and Problem-Solving
Common problems encountered in recombinant protein production and strategies for troubleshooting and problem-solving

Module #16
Expression Systems for Difficult-to-Express Proteins
Specialized expression systems for producing difficult-to-express proteins, including membrane proteins and glycoproteins

Module #17
Cell-Free Protein Synthesis
Overview of cell-free protein synthesis systems and their applications

Module #18
Animal-Derived Component-Free (ADCF) Production
Strategies for ADCF production, including serum-free media and animal-derived component-free reagents

Module #19
Single-Use Technologies in Recombinant Protein Production
Advantages and applications of single-use technologies in recombinant protein production

Module #20
Regulatory Aspects of Recombinant Protein Production
Regulatory requirements and guidelines for recombinant protein production, including FDA and EMA regulations

Module #21
Safety Considerations in Recombinant Protein Production
Safety considerations and biosafety protocols in recombinant protein production

Module #22
Economic and Environmental Impact of Recombinant Protein Production
Economic and environmental impact of recombinant protein production, including cost analysis and sustainability

Module #23
Emerging Trends and Technologies in Recombinant Protein Production
Overview of emerging trends and technologies in recombinant protein production, including gene editing and synthetic biology

Module #24
Case Studies in Recombinant Protein Production
Real-world examples and case studies of recombinant protein production, including successful products and challenges overcome

Module #25
Course Wrap-Up & Conclusion
Planning next steps in Recombinant Protein Production career

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Recombinant Protein Production ( 25 Modules )

Recombinant Protein Production
( 25 Modules )