Techniques in Molecular Biology: PCR, Cloning, and Sequencing
( 25 Modules )

Module #1
Introduction to Molecular Biology Techniques
Overview of molecular biology techniques, importance in research and diagnostics

Module #2
Principles of PCR (Polymerase Chain Reaction)
Basic principles, history, and applications of PCR

Module #3
PCR Instrumentation and Cycle Parameters
PCR machine components, thermal cycling, and parameter optimization

Module #4
PCR Primer Design and Validation
Primer design principles, primer validation, and common pitfalls

Module #5
PCR Optimization and Troubleshooting
Common PCR problems, optimization strategies, and troubleshooting techniques

Module #6
Real-Time PCR (qPCR) and its Applications
Principles, advantages, and applications of real-time PCR

Module #7
Introduction to Cloning
Overview of cloning, importance in molecular biology, and types of cloning vectors

Module #8
Restriction Enzymes and Cloning Strategies
Restriction enzymes, cloning strategies, and vector design

Module #9
Ligation and Transformation
Ligation reactions, transformation methods, and competent cell preparation

Module #10
Cloning Vector Systems
Overview of common cloning vectors, including plasmids, cosmids, and BACs

Module #11
Cloning and Subcloning
Cloning and subcloning strategies, including PCR-based cloning and Gibson assembly

Module #12
DNA Sequencing Fundamentals
History, principles, and applications of DNA sequencing

Module #13
Sanger Sequencing and Related Techniques
Sanger sequencing, dideoxy sequencing, and related methods

Module #14
Next-Generation Sequencing (NGS) Platforms
Illumina, PacBio, and Oxford Nanopore sequencing platforms

Module #15
NGS Library Preparation and Sequencing Strategies
Library preparation methods, including PCR-free and amplicon-based approaches

Module #16
Sequence Analysis and Bioinformatics
Sequence assembly, annotation, and analysis using bioinformatics tools

Module #17
Applications of PCR, Cloning, and Sequencing in Research and Diagnostics
Case studies and examples of molecular biology techniques in various fields

Module #18
PCR-Based Diagnostics and Clinical Applications
PCR-based diagnostics, including infectious disease detection and genetic testing

Module #19
Gene Expression Analysis using PCR and Sequencing
Gene expression analysis using PCR, qPCR, and RNA-seq

Module #20
Molecular biology Techniques in Forensic Science
Applications of PCR, cloning, and sequencing in forensic science

Module #21
Ethical Considerations in Molecular Biology
Ethical implications of molecular biology techniques, including genetic testing and gene editing

Module #22
Laboratory Safety and Quality Control
Laboratory safety guidelines, quality control measures, and troubleshooting common issues

Module #23
Advanced Topics in PCR and Cloning
Advanced PCR techniques, including multiplex PCR and digital PCR, and advanced cloning strategies

Module #24
Advanced Topics in Sequencing and Bioinformatics
Advanced sequencing technologies, including single-molecule sequencing, and advanced bioinformatics tools

Module #25
Course Wrap-Up & Conclusion
Planning next steps in Techniques in Molecular Biology: PCR, Cloning, and Sequencing career

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Techniques in Molecular Biology: PCR, Cloning, and Sequencing ( 25 Modules )

Techniques in Molecular Biology: PCR, Cloning, and Sequencing
( 25 Modules )