Techniques in Protein Purification
( 25 Modules )

Module #1
Introduction to Protein Purification
Overview of protein purification, importance, and challenges

Module #2
Protein Purification Strategies
Understanding protein properties, purification goals, and selection of purification strategies

Module #3
Cell Disruption and Lysis
Methods for breaking open cells to release proteins, including mechanical, chemical, and enzymatic methods

Module #4
Centrifugation and Sedimentation
Principles and applications of centrifugation and sedimentation in protein purification

Module #5
Filtration and Microfiltration
Methods for separating proteins based on size, including dead-end filtration and cross-flow filtration

Module #6
Clarification and Concentration
Methods for clarifying and concentrating protein samples, including ultrafiltration and diafiltration

Module #7
Chromatography Overview
Introduction to chromatography, types of chromatography, and principles of separation

Module #8
Gel Filtration Chromatography
Principles and applications of gel filtration chromatography, also known as size exclusion chromatography

Module #9
Ion Exchange Chromatography
Principles and applications of ion exchange chromatography, including cation and anion exchange

Module #10
Hydrophobic Interaction Chromatography
Principles and applications of hydrophobic interaction chromatography, including hydrophobic and ionic interactions

Module #11
Affinity Chromatography
Principles and applications of affinity chromatography, including antibody-based and ligand-based affinity purification

Module #12
Reversed Phase Chromatography
Principles and applications of reversed phase chromatography, including hydrophobic and hydrophilic interactions

Module #13
High-Performance Liquid Chromatography (HPLC)
Principles and applications of HPLC, including instrumentation and method development

Module #14
Gel Electrophoresis
Principles and applications of gel electrophoresis, including SDS-PAGE and native PAGE

Module #15
Western Blotting
Principles and applications of western blotting, including transfer techniques and detection methods

Module #16
Protein Detection and Quantification
Methods for detecting and quantifying proteins, including colorimetric, fluorescent, and chemiluminescent assays

Module #17
Protein Purity Analysis
Methods for analyzing protein purity, including SDS-PAGE, western blotting, and mass spectrometry

Module #18
Scaling Up Protein Purification
Considerations for scaling up protein purification, including bioreactor design and process optimization

Module #19
Protein Purification in Industry
Applications of protein purification in industrial settings, including biotechnology and pharmaceutical industries

Module #20
Current Advances in Protein Purification
Recent advances and emerging trends in protein purification, including new chromatography resins and techniques

Module #21
Quality Control and Validation
Importance of quality control and validation in protein purification, including regulatory considerations

Module #22
Troubleshooting Protein Purification
Common issues and troubleshooting strategies in protein purification, including contamination and aggregation

Module #23
Computational Tools in Protein Purification
Applications of computational tools in protein purification, including protein structure prediction and molecular modeling

Module #24
Case Studies in Protein Purification
Real-world examples of protein purification, including successful strategies and challenges overcome

Module #25
Course Wrap-Up & Conclusion
Planning next steps in Techniques in Protein Purification career

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Techniques in Protein Purification ( 25 Modules )

Techniques in Protein Purification
( 25 Modules )