Tissue Culture Methods for Plants
( 25 Modules )

Module #1
Introduction to Plant Tissue Culture
Overview of plant tissue culture, its importance, and applications

Module #2
History of Plant Tissue Culture
Evolution of plant tissue culture, key milestones, and contributions of pioneers

Module #3
Basic Principles of Plant Tissue Culture
Understanding plant growth and development, cell biology, and tissue organization

Module #4
Laboratory Setup and Equipment
Designing a tissue culture laboratory, equipment, and instrumentation

Module #5
Media Preparation and Sterilization
Types of media, media preparation, and sterilization techniques

Module #6
Explant Selection and Preparation
Choosing the right explant, surface sterilization, and preparation techniques

Module #7
Initiation of Tissue Culture
Inoculation, incubation, and initial growth of explants

Module #8
Callus Culture and Subculturing
Callus induction, maintenance, and subculturing techniques

Module #9
Organogenesis and Embryogenesis
Differentiation of callus into organs and embryos, factors influencing organogenesis and embryogenesis

Module #10
Micropropagation and In Vitro Regeneration
Clonal propagation, in vitro plantlet regeneration, and acclimatization

Module #11
Rooting and Acclimatization
Root induction, root development, and acclimatization of in vitro plants

Module #12
Tissue Culture for Crop Improvement
Applications of tissue culture in crop improvement, genetic engineering, and mutagenesis

Module #13
Tissue Culture for Conservation of Endangered Species
Role of tissue culture in conservation of endangered plant species, cryopreservation, and conservation biotechnology

Module #14
Disease Diagnostics and Elimination
Detection and elimination of plant diseases using tissue culture, indexing, and thermotherapy

Module #15
Somaclonal Variation and Its Applications
Somaclonal variation, its causes, and applications in crop improvement and biotechnology

Module #16
Scale-up and Commercialization
Large-scale micropropagation, commercialization, and economic considerations

Module #17
Quality Control and Contamination Management
Quality control measures, contamination management, and troubleshooting

Module #18
Troubleshooting in Tissue Culture
Common problems, troubleshooting, and optimization of tissue culture protocols

Module #19
Molecular Biology Techniques in Tissue Culture
Applications of molecular biology techniques, such as PCR and DNA sequencing, in tissue culture

Module #20
Bioinformatics and Computational Tools
Bioinformatics and computational tools for genomics, transcriptomics, and proteomics in plant tissue culture

Module #21
Case Studies in Plant Tissue Culture
Real-life examples and case studies of successful plant tissue culture applications

Module #22
Regulations and Intellectual Property Rights
Regulatory frameworks, intellectual property rights, and patenting in plant tissue culture

Module #23
Future Directions and Emerging Trends
Advances in single-cell technology, gene editing, and synthetic biology in plant tissue culture

Module #24
Hands-on Training and Practical Exercises
Practical exercises and hands-on training in tissue culture laboratory

Module #25
Course Wrap-Up & Conclusion
Planning next steps in Tissue Culture Methods for Plants career

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Tissue Culture Methods for Plants ( 25 Modules )

Tissue Culture Methods for Plants
( 25 Modules )